HPLC UV Method for Analysis of Blasticidine S on Chromni Column
https://sielc.com/hplc-uv-analysis-of-blasticidin-sChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Blasticidin-S
Blasticidin S is an antibiotic with the chemical formula C 17 H 26 N 8 O 5 . It is primarily used in biology research for selecting cells of interest among cell cultures. It works through disrupting protein translation within human cells, fungi, and bacteria due to it’s resemblance to cytidine. You can find detailed UV spectra of Blasticidin-S and information about its various lambda maxima by visiting the following link.
Blasticidin-S can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocraticmethod with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 70/30 |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275nm |
| Limit of Detection* | 3.1 ppb |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Blasticidin-S |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended