HPLC Method for Separation of Sulfur-containing Biomolecules on Primesep 100 Column
https://sielc.com/hplc-separation-sulfur-containing-biomoleculesChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
HPLC Method for Separation of Cysteine, Glutathione, reduced, Cystine, Cysteine-glutathione disulfide, Glutathione oxidized on Primesep 100 Column by SIELC Technologies
These compounds are all involved in redox reactions and cellular defenses against oxidative stress in biological systems. Here’s a bit more about each of them:
Overall, these molecules play vital roles in the body’s antioxidant defenses, detoxification of xenobiotics, modulation of redox-controlled signaling pathways, and regulation of cellular proliferation and apoptosis.
These compounds can be retained, separated, and analyzed using a reverse-phase Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Sulfuric acid, which serves as a buffer. This analytical method can be
Method Parameters
| Mobile Phase | MeCN -10% |
|---|---|
| Buffer | Gradient H2SO4 0.1-0.3%, 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Thiol, Amino acid |
| Analyzing Compounds | Cysteine, Glutathione, reduced, Cystine, Cysteine-glutathione disulfide, Glutathione oxidized |