HPLC Separation of Uridine Sugar Diphospates on Primesep N Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Gadolinium-DOTA
Uridine sugar diphospates are a class of complex nucleotide sugars that are integral to metabolic processes in the cell. Uridine diphosphate galactose (UDP-Galactose), Uridine diphosphate glucose (UDP-Glucose), and uridine diphosphate N-acetylglucosamine (UDP-N-acetylgalactoseamine or UDP-GlcNAc) are some of the most well-known nucleotide sugars.
Uridine Diphosphate Glucose ( UDP-glucose ) is an an important nucleotide sugar with the chemical formula C 15 H 24 N 2 O 17 P 2 . It is involved in metabolic processes in the cell. It is a precursor to glycogen, UDP-galactose, and UDP-glucuronic acid, as well as other polysacchrides and glycosphingolipids.
Uridine Diphosphate Galactose ( UDP-galactose ) is an intermediary formed in the biosynthesis of polysaccharides with the chemical formula C 15 H 24 N 2 O 17 P 2 . It is important in glycolysis, which is a metabolic pathway that converts glucose into pyruvate.
These three uridine sugar diphosphate compounds can be detected in the low UV regime. Using a Primesep N normal-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose, UDP-glucose, and UDP-GIcNAc can be retained, separated, and analyzed. This analysis method can be UV detected at 260 nm.
Method Parameters
| Mobile Phase | MeCN – 80% |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Class of Compounds | Sugar, Phospate |
| Analyzing Compounds | Uridine-5'-diphosphate-glucose,Uridine 5'-diphosphogalactose disodium salt,Uridine 5′-diphospho-N-acetylgalactosamine |