HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH
https://sielc.com/hplc-separation-of-uracil-thymine-guanine-cytosine-adenine-on-newcrom-ahChromatogram
HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH Column_1245
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Uracil , Thymine , Adenine , Cytosine , Guanine .
Method Parameters
| Mobile Phase | H2O – 100% |
|---|---|
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm, MS-compatible mobile phase |
| Class of Compounds | Hydrophilic, Drug, Xanthine, Nucleic Bases |
| Analyzing Compounds | Uracil,Thymine,Adenine,Cytosine,Guanine |
HPLC Column Used
Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended