HPLC ELSD Method for Analysis of Triethanolamine on Primesep 500 Column

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Triethanolamine

Triethanolamine , also denoted as TEOA , is an organic compound with the chemical formula N(CH2CH2OH)3 . It is primarily used in making surfactants both for industrial and commercial use. It is also found in sunscreen, lotions, liquid detergents, sanitizers, and inks. You can find detailed UV spectra of Triethanolamine and information about its various lambda maxima by visiting the following link.

Triethanolamine can be retained and analyzed using the Primesep 500 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Acetate as a buffer. Detection is performed using ELSF.

Method Parameters

Mobile PhaseMeCN/H2O – 10/90 %
BufferAmmonium Acetate pH 4.0 – 20 mM
Flow Rate0.2 ml/min
DetectionELSD
Limit of Detection*7ppm
Class of CompoundsOrganic Compound
Analyzing CompoundsTriethanolamine

HPLC Column Used

Primesep 500, 2.1 x 150 mm, 5 µm, 100 A, dual ended