HPLC Separation of Ten Cannabinoids on Cannsep C Column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetrahydrocannabinol (THC) , Delta-9-Tetrahydrocannabinol (THC) , Tetrahydrocannabinolic acid (THCA-A) , Tetrahydrocannabivarin (THCV) , Cannabidiol (CBD) , Cannabinol (CBN) , Cannabichromene (CBC) , Cannabidivarin (CBDV) , Cannabigerol (CBG) , Cannabidiolic acid (CBDA) .

Cannabidivarin ( CBDV ) is a non-intoxicating psychoactive cannabinoid with the chemical formula C 19 H 26 O 2 . Due to it demonstrating anticonvulsive properties, it is actively in Phase-2 trials for adult epilepsy, childhood epilepsy, and for Prader-Willi Syndrome.

Tetrahydrocannabivarin ( THCV , THV ) is a cannabinoid receptor type 1 antagonist with the chemical formula C 19 H 26 O 2 . It is prevalent in certain strains of Cannabis in central Asia and southern Africa. It is currently being researched as a potential treatment for obesity-associated glucose intolerances. It is also said to reduce appetite and have energizing effects.

Cannabidiol ( CBD ) is a phytocannabinoid with the chemical formula C 21 H 30 O 2 . It is used in a variety of ways. It’s only confirmed medical use is as epilepsy medication. It may help with pain, sleep, and addiction as an alternative to opioids, but due to limited clinical evidence, it’s regulations are dependent on the state. It is advised against using it during pregnancy due to unknown effects on fetal and infant development.

Cannabidiolic Acid ( CBDA ) is a precursor to Cannabidiol with the chemical formula C 22 H 30 O 4 . Research suggests that it might have anti-inflammatory, anti-emetic, anti-convulsant, and anti-carcinogenic properties, but not enough research has been done into it for it to be used as widely as other cannabinoids and the like.

Cannabigerol ( CBG ) is a non-psychoactive cannabinoid with the chemical formula C 21 H 32 O 2 .  It is a minor constituent of cannabis. CBG that is derrived from marijuana is illegal, while CBG derived from hemp is legal as long as the THC content of hemp is less than 0.3% of dry weight. It was sold illegally by certain companies under unproven claims of being treatment against the COVID-19 virus and inflammation.

Cannabinol ( CBN ) is a mildly psychoactive phytocannabinoid with the chemical formula C 21 H 26 O 2 . It was the first cannabinoid to be isolated from cannabis in the late 1800s. It is legal in the United States as long as the THC concentration is 0.3% or less.

Method Parameters

HPLC Column Used