HPLC Method for Separation of Serine, a-Methylserine, GABA, and Methionine Mixture on Primesep 100 column
https://sielc.com/application-hplc-separation-of-serine-a-methylserine-gaba-and-methionine-mixtureChromatogram
Description
Four underivatized amino acids (serine, methylserine, GABA and methionine) were separated on a Primesep 100 reversed-phase cation-exchange mixed-mode HPLC column. Two methods, one for UV and one for ELSD/LC/MS show good separation and peak shape for underivatized amino acids. This column and general HPLC approach can be used for analysis of underivatized amino acids. Primesep 100 is designed to replace reversed-phase HPLC column in combination with ion-pairing reagents.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H3PO4 or Formic acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm, ELSD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Serine, Methylserine, GABA, Methionine |
HPLC Column Used
Primesep 100, 4.6×250 mm, 5 µm, 100A