HPLC Separation of Pyrazinecarboxamide and Related Compounds

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyrazine , 2-Aminopyrazine , Pyrazinamide , 3-Aminopyrazole-4-carboxylic acid .

Pyrazinamide is a medication used in the treatment of infectious disease tuberculosis and is on the World Health Organization’s (WHO) list of essential medicines. It has the chemical formula C 5 H 5 N 3 O .

Pyrazine is an organic compound with C₄H₄N₂ chemical formula. It is known for its sweet and nutty aroma, but is usually used as a therapeutic agent in several drugs. Derivatives of it are also said to have anti-inflammatory, anticancer, antibacterial, and antioxidant properties.

Aminopyrazine, also known as 2-aminopyrazine and pyrazinamide, is a Pyrazine derivative with C₄H₅N₃ chemical formula. It is typically used as a building block in chemical synthesis and is found in pharmaceuticals, especially  in antiviral drugs and other agrochemicals. Structure wise, it is a pyrazine ring with an attached amine group.

It can be retained and separated from other pyrazine compounds, which are structurally similar and can be difficult to separate in reverse-phase HPLC, by using Primesep A mixed-mode column. Primesep A’s stationary phase is embedded with strong acidic ion-pairing groups. The analytical method is isocratic and uses the mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) as buffer and UV detection at 275nm.

Method Parameters

HPLC Column Used