HPLC Separation of Pyrazine and Aminopyrazine on Sharc 1 Column
https://sielc.com/hplc-separation-of-pyrazine-and-aminopyrazineChromatogram
HPLC Separation of Pyrazine and Aminopyrazine on Sharc 1 Column_1198
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyrazine , 2-Aminopyrazine
Pyrazine is an organic compound with C₄H₄N₂ chemical formula. It is known for its sweet and nutty aroma, but is usually used as a therapeutic agent in several drugs. Derivatives of it are also said to have anti-inflammatory, anticancer, antibacterial, and antioxidant properties.
Aminopyrazine, also known as 2-aminopyrazine and pyrazinamide, is a Pyrazine derivative with C₄H₅N₃ chemical formula. It is typically used as a building block in chemical synthesis and is found in pharmaceuticals, especially in antiviral drugs and other agrochemicals. Structure wise, it is a pyrazine ring with an attached amine group.
Pyrazine , 2-Aminopyrazine can be retained and analyzed using the SHARC 1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 98/2% |
|---|---|
| Buffer | Formic Acid – 0.5 % AmFm -0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| Class of Compounds | Hydrophilic, Heterocyclic, Aromatic |
| Analyzing Compounds | Pyrazine,2-Aminopyrazine |