HPLC Separation of Procymidone on Newcrom B Column
https://sielc.com/hplc-separation-of-procymidone-on-newcrom-b-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Procymidone .
Procymidone is a pesticide and fungicide with the chemical formula C 13 H 11 Cl 2 NO 2 . It is typically used to kill unwanted ferns, nettles, and fungi in lupins, grapes, stone fruit, and strawberries.
Procymidone can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | Formic Acid – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 260 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Procymidone |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended