HPLC Separation of Phthalates

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Diethylphthalate (DEP) , Dimethylphthalate (DMP) , Dipropylphthalate (DPP) , Dibutylphthalate (DBP) .

Dimethyl Phthalate (DMP) is a phthalate ester with the chemical formula C 10 H 10 O 4 .  It has a vide variety of uses from being an ingredient in insect repellent to being a part of safety glass and goggles.  While it may appear similar to water, it is only soluble in organic solvents and not water. DMP is an irritant to eyes, nose, and throat through inhalation.

Diethyl Phthalate (DEP) is a phthalate ester with the chemical formula C 10 H 10 O 4 .  It is used as a solvent in perfumes, lotions, and creams. In medicine, it is used as an inactive ingredient for purposes of slowing down the release of active ingredients.

Dipropyl Phthalate (DPP) is a phthalate with the chemical formula C 14 H 18 O 4 .  It is used to increase the durability elasticity of plastics while also being a solvent for certain chemical applications. While it’s toxicity is low, it can still be dangerous, considering the risks of phthalates.

Dibutyl Phthalate (DBP) is a phthalate with the chemical formula C 16 H 22 O 4 .  It is used to increase the durability elasticity of plastics, as well as an ingredient in glues. DBP is an endocrine disruptor, which means that it effects hormone production.

Diethylphthalate (DEP) , Dimethylphthalate (DMP) , Dipropylphthalate (DPP) , Dibutylphthalate (DBP) can be retained and analyzed using the Newcrom A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.

Method Parameters

HPLC Column Used