HPLC Method for Analysis of Neopterin on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Neopterin .
Neopterin is an pteridine with the chemical formula C 9 H 11 N 5 O 4 . It is indicative of a pro-inflammatory immune status, therefore it typically serves as a marker of the activation of the cellular immune system. You can find detailed UV spectra of Neopterin and information about its various lambda maxima by visiting the following link.
Neopterin retained and analyzed using a reverse-phase Primesep 100 column. The mobile phase for this method consists of water, acetonitrile (MeCN), and ammonium formate, which serves as a buffer. This analytical method can be monitored using UV detection at 275 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method such as Charged Aerosol Detection (CAD) or Electrospray Ionization Mass Spectrometry (ESI-MS)
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Method Parameters
| Mobile Phase | MeCN/H2O -5/95% |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Peak Retention Time | 3.28 min |
| Sample concentration | 0.4 mg/ml |
| Injection volume | 1 µl |
| Sample diluent | H2O + NaOH |
| LOD | 10 ppb |
| Class of Compounds | Pteridines |
| Analyzing Compounds | Neopterin |