HPLC Separation of NAD and NADH on PEI Column
https://sielc.com/hplc-separation-of-nad-and-nadh-on-pei-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of SNAD and NADH
Nicotinamide adenine dinucleotide (NAD), is a coenzyme found in every single living cell. NAD can exist in two forms: NAD+ and NADH. The conversion of NAD from its oxidized form (NAD+) to its reduced form (NADH), and back, provides the cell with a mechanism for accepting and donating electrons.
NAD and NADH can be retained, separated and UV detected at 260 nm using the PEI column with a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV, ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Ammonium Acetate pH 6.8 – 60 mM |
| Flow Rate | 2.0 ml/min |
| Detection | UV 260 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Nicotinamide Adenine Dinucleotide (NAD),Nicotinamide Adenine Dinucleotide (reduced) (NADH) |
HPLC Column Used
PEI, 4.6×150 mm, 5 µm, 100A