HPLC Separation Of Mixture Of Pyridylacetic Acids (2-Pyridylacetic acid, 3-Pyridylacetic acid, 4-Pyridylacetic acid) On Primesep N Column
https://sielc.com/hplc-separation-of-mixture-of-pyridylacetic-acidsChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride , 3-Pyridylacetic acid hydrochloride , 4-Pyridylacetic acid hydrochloride
Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.
2-Pyridylacetic acid hydrochloride , 3-Pyridylacetic acid hydrochloride , 4-Pyridylacetic acid hydrochloride can be retained and analyzed using the Primesep N stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | AmFm pH 4.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Metabolite of nicotine and other tobacco alkaloids |
| Analyzing Compounds | 2-Pyridylacetic acid hydrochloride3-Pyridylacetic acid hydrochloride4-Pyridylacetic acid hydrochloride |