HPLC Separation Of Mixture of Pyridylacetic Acids And Their Derivatives
https://sielc.com/hplc-separation-of-mixture-of-pyridylacetic-acids-and-their-derivativesChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Pyridylacetic acid hydrochloride , 3-Pyridylacetic acid hydrochloride , 4-Pyridylacetic acid hydrochloride , Nicotinic Acid/Niacin (3-pyridinecarboxylic acid) , 3-Acetylpyridine , Pyridyl-3-thioacetomorpholide
Pyridylacetic Acids are a class of compounds with a pyridine ring and an acetic acid. These acids are typically building blocks found in organic synthesis/ On occasion, they can also act as human xenobiotic metabolites.
Nicotinic acid , also known as niacin and vitamin B3, is a water-soluble B vitamin with C₆H₅NO₂ molecular formula. It is crucial for energy production.
Derrivatives are compounds formed when the parent compound goes through a chemical recation, replacing atoms with different ones, leading to different physical and chemical properties.
2-Pyridylacetic acid hydrochloride , 3-Pyridylacetic acid hydrochloride , 4-Pyridylacetic acid hydrochloride , Nicotinic Acid/Niacin (3-pyridinecarboxylic acid) , 3-Acetylpyridine , Pyridyl-3-thioacetomorpholide can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O Gradient – 5-50% |
|---|---|
| Buffer | CH2O2 – 0.02-0.05% |
| Flow Rate | 1.0ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Acids |
| Analyzing Compounds | 2-Pyridylacetic acid hydrochloride,3-Pyridylacetic acid hydrochloride,4-Pyridylacetic acid hydrochloride,Nicotinic Acid/Niacin (3-pyridinecarboxylic acid),3-Acetylpyridine,Pyridyl-3-thioacetomorpholide |