HPLC Separation of a Mixture of Non-Essential Amino Acids, such as L-Aspartic Acid, L-Serine, L-Glutamic Acid, and L-Alanine on Primesep 100 Column
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HPLC Separation of Mixture of Non-Essential Amino Acids on Primesep 100 Column
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Aspartic Acid,Serine,GLU (L-Glutamic acid),Glutamic Acid,D-Alanine,DL-Alanine,Amino Acids,Alanine |
HPLC Column Used
Primesep 100, 4.6 x 250 mm, 5 µm, 100 A, dual ended