HPLC Separation of Mixture of Mycophenolate mofetil and Mycophenolic Acid on Newcrom R1 Column
https://sielc.com/hplc-separation-of-mixture-of-mycophenolate-mofetil-and-mycophenolic-acidChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Mycophenolic acid , Mycophenolate mofetil .
Mycophenolate mofetil and mycophenolic acid alongside other medicines are used as immunosuppressants to prevent organ rejection after a transplant. Both compounds can be retained and separated in HPLC using Newcrom R1 reverse-phase column. The analytical method’s mobile phase consists of a gradient of acetonitrile (ACN) in water with phosphoric acid (H3PO4) buffer and using UV detection at 220nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H3PO4- 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds | Drug, Acid, Hydrophobic, Ionizable, Immunosuppressant |
| Analyzing Compounds | Mycophenolic acid,Mycophenolate mofetil |
HPLC Column Used
Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended