HPLC Separation of Mixture of DMF and DMSO
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of DMF (Dimethylformamide) , DMSO (Dimethyl sulfoxide) .
Dimethylformamide, DMF, is primarily used as an organic solvent and is miscible with water and most other organic liquids. Dimethyl sulfoxide (DMSO) is also a solvent. It can dissolve both polar and nonpolar compounds and is miscible with water and other organic liquids. Both compounds can be retained and separated using anhydrous (water-free) conditions using HPLC by SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase without the need for a buffer. UV detection used at 220nm.
Method Parameters
| Mobile Phase | Gradient MeCN – 98-85 %, 10 min |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds | Drug, Basic, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | DMF (Dimethylformamide),DMSO (Dimethyl sulfoxide) |
HPLC Column Used
SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended