HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column
https://sielc.com/hplc-separation-of-mixture-of-conditionally-essential-amino-acidsChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Glutamine , Glycine , Cysteine , L-Cysteine , Proline , Tyrosine , Arginine , Amino Acids .
Glutamine is an amino acid with the chemical formula C 5 H 10 N 2 O 3 . It is a building block for protein, but it also supports the immune system, gut health, and detoxification. It is usually found in meat, fish, eggs, dairy, and whole grains; though some consider it the most abundant amino acid in the body. People also take glutamine as treatment for sickle cell disease, healing of burns, recovery after surgery, and injuries.
L-Glycine is an important amino acid compounds widely used in pharmaceutical, biochemical, and peptide research. It has he chemical formula C₂H₅NO₂ . It is water-soluble and plays a critical role in protein synthesis, peptide modification, and metabolic studies. It can be found in meat., eggs. and bones.
L-Cysteine is an amino acid with the chemical formula C 5 H 10 N 2 O 3 . It is primarily a building block for protein, but it also has antioxidant effects. On occasion, ot os used to support people dealing with cancer, diabetes, and hangover, but there is yet to be substantial evidence that it works. Poultry, egg, beef, and whole grains are rich sources of the amino acid.
L-Proline is an amino acid with the chemical formula C 5 H 9 NO 2 . It is naturally produced in the body, but it is also recommended to be consumed through the diet. It can be found in meat, fish, and dairy. It is used for skin healing and treatment of other skin conditions as it is an essential component of collagen. It is important for upkeep of joints and tendons, as well as heart muscles.
L-Tyrosine is an amino acid with the chemical formula C 9 H 11 NO 3 . It is naturally used in the body to create neurotransmitters like dopamine and norepinephrine. While it is produced in the body naturally, it can also be consumed in beef, pork, fish, dairy products, and beans. As a supplement, it is also used to treat Phenylketonuria (PKU).
L-Arginine is an amino acid with the chemical formula C 9 H 11 NO 3 . It is a precursor for nitric acid, making it crucial for blood flow and general cardiovascular health. It can help lower blood pressure, help with chest pain, and improve symptoms of Peripheral arterial disease (PAD).
Method Parameters
| Mobile Phase | MeCN/H2O – 35/65% |
|---|---|
| Buffer | H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | L-Glutamine,Glycine,Cysteine,L-Cysteine,Proline,Tyrosine,Arginine,Amino Acids |