HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid on Primesep B Column
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HPLC Separation of Mixture of 2-Hydroxyethyl Salicylate and Salicylic Acid_1182
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Hydroxyethyl salicylate , Salicylic acid
2-Hydroxyethyl salicylate is a drug used as analgesic agents for the treatment of mild to moderate pain.
2-Hydroxyethyl salicylate , Salicylic acid can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 25/75% |
|---|---|
| Buffer | H2SO4 – 0.5 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 238 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Carboxylic acid, Drug, Carbocyclic, Benzoate, Hydroxy Acid, Hydroxybenzoate, Phenol |
| Analyzing Compounds | 2-Hydroxyethyl salicylate,Salicylic acid |
HPLC Column Used
Primesep B