HPLC Method for Analysis of Ibotenic Acid on Primesep A Column
https://sielc.com/hplc-separation-of-ibotenicChromatogram
Description
Ibotenic acid , also known as ibotenate , premuscimol or as (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetic acid , is an a -amino acid with the chemical formula C 5 H 6 N 2 O 4 . It is a metabotropic glutamate receptor agonist found in certain Amanita mushrooms. It is known for it’s neurotoxic properties. You can find detailed UV spectra of Ibotenic acid and information about its various lambda maxima by visiting the following link.
Ibotenic acid can be retained, separated, and analyzed on a mixed-mode Ibotenic acid column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 4.12 min |
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic acid |
HPLC Column Used
Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended