HPLC Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A Column
https://sielc.com/hplc-separation-of-ibotenic-glutamic-2Chromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode · Method for Separation of Ibotenic Acid and Glutamic Acid on Primesep A by SIELC Technologies
Ibotenic acid is a physchoactive NDMA and metabotropic glutamate receptor agonist with neurotoxic properties. These structurally similar psychoactive drugs can be retained, separated, and analyzed on a mixed-mode Primesep A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peak Retention Time | 3.22 min, 10.52 min |
| Class of Compounds | Acid |
| Analyzing Compounds | Ibotenic Acid, Glutamic Acid |
HPLC Column Used
Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended