HPLC Method for Separation of Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column
https://sielc.com/hplc-separation-of-histidine-bistChromatogram
Description
· Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
HPLC Method for Separation of of Histidine, Histidine Methyl Ester and Histidine lauryl Ester on BIST B+ Column by SIELC Technologies
Histidine is a naturally occurring essential amino acid that the body uses to repair damaged tissue and generate new blood cells. Histidine Methyl Ester is a histidine decarboxylase inhibitor and can be used to synthesize other biological compounds. Histidine Lauryl Ester is another derivative of Histidine. Using SIELC’s newly introduced BIST™ method, Histidine and its derivatives can be retained and separated on a positively charged, anion-exchange BIST™ B+ column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively charged dipeptide to the positively charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Histidine and its derivatives can be UV detected at 220 nm.
Method Parameters
| Mobile Phase | Gradient MeCN – 80 – 50%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 220 nm |
| Peak Retention Time | 2.2 min, 7.5 min, 8.9 min |
| Class of Compounds | Amino acid |
| Analyzing Compounds | Histidine methyl ester,Histidine |