HPLC Method for Analysis of Glutathione reduced (GSH) on Primesep 100 Column

Chromatogram

Description

· HPLC Method for Analysis of Glutathione on Primesep 100 .

L-Glutathione , also known as reduced glutathione or simply GSH , is a tripeptide made up of three amino acids: L-glutamic acid, L-cysteine, and glycine. It has the chemical formula C 10 H 17 N 3 O 6 S .

Glutathione is found in nearly all cells, and in its reduced form (GSH), it is a powerful antioxidant. It plays a crucial role in protecting cells against oxidative stress by neutralizing reactive oxygen species such as free radicals and peroxides.

The unique aspect of glutathione is the cysteine thiol group (SH) which acts as a reducing agent and can be reversibly oxidized and reduced. In cells, glutathione is maintained in the reduced form by the enzyme glutathione reductase, which reduces the disulfide form of the molecule (GSSG, glutathione disulfide) back to GSH.

Apart from being an antioxidant, glutathione also plays other important roles including detoxification, immune system support, protein function regulation, and as a cofactor for various enzymes. Its deficiency can lead to cellular stress and dysfunction, underscoring its vital role in maintaining cellular health.

In supplement form, L-Glutathion e is often used with the intent to support antioxidant activity in the body. However, it is important to consult with a healthcare provider before starting any new supplement regimen.

Glutathione can be retained and analyzed using a reverse-phase Primesep 100 column. The mobile phase for this method consists of water, acetonitrile (MeCN), and sulfuric acid, which serves as a buffer. This analytical method can be monitored using UV detection at 200 nm.

Method Parameters

HPLC Column Used