HPLC Separation of Enalapril and Lisinopril on Newcrom AH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Enalapril and Lisinopril
Enalapril is an ACE inhibitor medication with the chemical formula C 20 H 28 N 2 O 5 . It is used to treat hypertension, symptomatic heart failure, and asymptomatic left ventricular dysfunction. It also exhibited the ability to reduce the progression of kidney disease. Generic versions of it began their production in 2000.
Lisinopril is a medications that work on the kidneys to help lower blood pressure. It can be used for heart failure and for people who’ve had a heart attack. Like Enalapril, it is an ACE inhibitor. It has the chemical formula C 21 H 31 N 3 O 5 .
By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, enalapril and lisinopril, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a sulfuric acid (H2SO4) buffer. UV detection at 210 nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Enalapril,Lisinopril,Lisinopril dihydrate |