HPLC Separation of EDTA, Oxymetazoline and Benzyl Alcohol in Nasal Spray
https://sielc.com/hplc-separation-of-edta-oxymetazoline-and-benzyl-alcohol-in-nasal-sprayChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol
Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.
Method Parameters
| Mobile Phase | Gradient MeCN – 2- 70%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260nm |
| Class of Compounds | Acid, Hydrophilic,Preservatives |
| Analyzing Compounds | EDTA (Ethylenediaminetetraacetic Acid),Oxymetazoline,Benzyl alcohol,Oxymetazoline hydrochloride |