HPLC Separation of DOTA and DO3A on Primesep 100 Column
https://sielc.com/hplc-separation-of-dota-and-do3aChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of DO3A , Tetraxetan (DOTA)
DOTA is a macrocyclic cheating agent with the chemical formula C 16 H 28 N 4 O 8 . DOTA is actually an acronym for 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid. It is used to bind metal ions, partially due to it’s stability. It is often employed in biomedical applications including but not limited to MRI contrast agents and targeted cancer therapy.
DO3A is a derivative of DOTA with the chemical formula C₁₄H₂₃N₄O₆Na₃.
DO3A , Tetraxetan (DOTA) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic. |
| Analyzing Compounds | DO3A,Tetraxetan (DOTA) |