HPLC Method for Separation of DOTA and DO3A MS- compatible mobile phase
https://sielc.com/hplc-separation-of-dota-and-do3a-ms-compatible-mobile-phaseChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Tetraxetan (DOTA) , DO3A
DOTA is a macrocyclic cheating agent with the chemical formula C 16 H 28 N 4 O 8 . DOTA is actually an acronym for 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid. It is used to bind metal ions, partially due to it’s stability. It is often employed in biomedical applications including but not limited to MRI contrast agents and targeted cancer therapy.
DO3A is a derivative of DOTA with the chemical formula C₁₄H₂₃N₄O₆Na₃.
Tetraxetan (DOTA) , DO3A can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using CAD.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Carboxylic acid, Carbocyclic. |
| Analyzing Compounds | Tetraxetan (DOTA),DO3A |