HPLC ELSD Method for Analysis of Diethylentriamine on Primesep 500 Column
https://sielc.com/hplc-separation-of-diethylentriamineChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Diethylentriamine
Diethylenetriamine , also denoted as dien or DETA , is an organic compound with the chemical formula HN(CH 2 CH 2 NH 2 ) 2 . It is primarily used in as a curing agent for epoxy resins.
Diethylentriamine can be retained and analyzed using the Primesep 500 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid as a buffer. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90 % |
|---|---|
| Buffer | Ammonium Acetate pH 3.0 – 20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD |
| Limit of Detection* | 1 ppm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Diethylentriamine |
HPLC Column Used
Primesep 500, 2.1 x 150 mm, 5 µm, 100 A, dual ended