HPLC Method for Analysis of Dicarbon Carboxylic Acids on Newcrom BH Column
https://sielc.com/hplc-separation-of-dicarbon-acidsChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
These four acids be separate and analyzed on a reverse-phase Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A, dual ended column with a mobile phase consisting of water and perchloric acid as a buffer modifier. This analysis method can be UV detected at 200 nm.
Method Parameters
| Mobile Phase | H2O -99.7% |
|---|---|
| Buffer | HClO4 – 0.3 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Dicarbon Carboxylic Acids |
| Analyzing Compounds | Oxalic Acid,Glycolic acid,Acetic Acid,Glyoxylic acid |
HPLC Column Used
Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A, dual ended