HPLC Separation of cis- and trans – 1,4 cyclohexanedicarboxylic Acid on Newcrom BH Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,4-Cyclohexanedicarboxylic acid .
1,4-Cyclohexanedicarboxylic acid is a pair of organic compounds with the chemical formula C 8 H 12 O 4 . It is typically used as a building block for polyesters and polyamides. Due to it improving hardness, flexibility, and impact strength of the previously mentioned compounds, it is used in automotive and construction industries. You can find detailed UV spectra of 1,4-Cyclohexanedicarboxylic acid and information about its various lambda maxima by visiting the following link.
1,4-Cyclohexanedicarboxylic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 1,4-Cyclohexanedicarboxylic acid |