HPLC Method for Analysis of Cerium(III) Nitrate on Newcrom AH Column

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Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Carbonic acid, cerium(3+) salt (3:2) , Cerium(III) nitrate hexahydrate .

Cerium(III) is a specific oxidation state of the element cerium, which is a lanthanide and a rare-earth metal. Cerium(III) compounds are often used in a variety of applications. For example, cerium(III) chloride (CeCl3) is a common starting material for the preparation of other cerium-containing compounds and is often used as a reducing agent in organic chemistry. Cerium(III) oxide (Ce2O3), also known as cerous oxide, is used in ceramics and to polish glass. Cerium(III) nitrate ( CeH 12 N 3 O 15 ) is often used in the laboratory as a cerium(III) source because it is relatively stable and easy to handle. It can be used in a variety of reactions, such as the preparation of other cerium compounds or as a catalyst or co-catalyst in organic reactions.

Carbonic acid, cerium(3+) salt (3:2) , Cerium(III) nitrate hexahydrate can be retained and analyzed on a mixed-mode Newcrom AH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium Formate. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Method Parameters

Mobile PhaseGradient MeCN -50-70%, 10 min
BufferGradient AmFm pH 3.0 – 30-60 mM, 10 min
Flow Rate1.0 ml/min
DetectionELSD, 50C
Peak Retention Time6.02 min
LOD0.2 ppm
Class of CompoundsMetal
Analyzing CompoundsCarbonic acid, cerium(3+) salt (3:2),Cerium(III) nitrate hexahydrate

HPLC Column Used

Newcrom AH, 4.6 x 100 mm, 5 µm, 100 A, dual ended