HPLC Separation of Bromoacetic and Chloroacetic Acids on Newcrom BH Column
https://sielc.com/hplc-separation-of-bromoacetic-and-chloroacetic-acidsChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Difluoroacetic acid , Dichloroacetic acid , Dibromoacetic acid , Acetic Acid , Bromoacetic acid , Chloroacetic acid , Bromochloroacetic acid .
Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer. UV detection at 200nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Difluoroacetic acid,Dichloroacetic acid,Dibromoacetic acid,Acetic Acid,Bromoacetic acid,Chloroacetic acid,Bromochloroacetic acid |
HPLC Column Used
Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended