HPLC Separation of Bromoacetic and Chloroacetic acids on Newcrom B Column
https://sielc.com/hplc-separation-of-bromoacetic-and-chloroacetic-acids-2Chromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromoacetic acid , Dibromoacetic acid , Dichloroacetic acid , Trichloroacetic acid .
Bromo – and chloro – acetic acids are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acid |
| Analyzing Compounds | Bromoacetic acid,Dibromoacetic acid,Dichloroacetic acid,Trichloroacetic acid |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended