HPLC Separation of Benzoic and 2-naphtalenesulfonic Acids

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoic Acid , 2-Naphthalenesulfonic Acid .

Benzoic Acid is an organic acid with antimicrobial properties.  It is typically used in cosmetics, food, and pharmaceuticals as a preservative against yeast and mold. As treatment, it is also used as a topical medicine of fungal skin infections. It can be naturally found in many berries and vegetable resins. With it’s chemical formula being C 7 H 6 O 2 , it is considered the simplest aromatic carboxylic acid. You can find detailed UV spectra of Benzoic Acid and information about its various lambda maxima by visiting the following link.

2-Naphthalenesulfonic Acid is an organic compound with the formula C 10 H 7 SO 3 H . It is used primarily in the production of dyes, but has also historically been industrially used for a it’s various chemical reactions.

Newcrom BH column is used for HPLC separation of weak and strong organic acids using a mixed-mode method.  Benzoic and naphthalenesulfonic acids are retained based on polar interaction mode and anion-exchange mode.  The isocratic separation is achieved very quickly on a short 3.2 x 50 mm column in under 3 minutes.  The mobile phase is acetonitrile (ACN) and water with sulfuric acid buffer.  UV detection at 275nm.

Method Parameters

HPLC Column Used