HPLC Method for Analysis of Benzocaine on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzocaine
Benzocaine is a local anesthetic with the chemical formula C 9 H 11 NO 2 . It is primarily used to treat pain in the mouth, threat, and ears. On occasion it is also used on sunburns and hemorrhoids. It is also commonly used as a ingredient ibn other medications. You can find detailed UV spectra of Benzocaine and information about its various lambda maxima by visiting the following link .
Benzocaine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80 % |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV |
| Limit of Detection* | 10 ppb |
| Class of Compounds | Amino Ester |
| Analyzing Compounds | Benzocaine |
HPLC Column Used
Primesep 200, 3.2 x 150 mm, 3 µm, 100 A, dual ended