HPLC Separation of β-Lactam Antibiotics such as Amoxicillin and Cephalexin on Primesep 100 Column
https://sielc.com/hplc-separation-of-b2-lactam-antibioticsChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cephalexin , Amoxicillin , Amoxicillin hydrate (1:3) .
Both amoxicillin and cephalexin are beta-lactam antibiotics. Beta-lactam antibiotics contain a beta-lactam ring in their molecular structure. As a group, these drugs are active against many gram-positive, gram-negative and anaerobic organisms. Amoxicillin is a prescription antibiotic with the molecular formula C 16 H 19 N 3 O 5 S . It is typically used to treat bacterial infections and is taken orally. Cephalexin has the chemical formula C 16 H 17 N 3 O 4 S .
Cephalexin , Amoxicillin , Amoxicillin hydrate (1:3) can be retained and baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a Sulfuric acid (H2SO4) buffer. UV detection at 250 nm.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA, H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Antibiotics, Hydrophobic, Ionizable |
| Analyzing Compounds | Cephalexin,Amoxicillin,Amoxicillin hydrate (1:3) |