HPLC Method for Separation of Benzonitrile, Toluene and Benzylamine on Primesep A Column
https://sielc.com/hplc-separation-of-aromaticsChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
These chemicals are widely used in organic synthesis. Please handle these substances with care, as they can have hazardous effects. Always refer to the safety data sheets for each chemical and adhere to recommended safety procedures.
These three compounds be separate and analyzed on a reverse-phase Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended column with a mobile phase consisting of water, Acetonitrile (MeCN) and Sulfuric acid as a buffer modifier. This analysis method can be UV detected at 210 nm.
LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Method Parameters
| Mobile Phase | MeCN/H2O -60/40% |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Peaks Retention Time | 2.9, 4.0, 10.2 min |
| Samples concentration(Benzonitrile, Toluene, Benzylamine) | 0.04, 0.05, 0.07 mg/ml |
| Injection volume | 3 µl |
| Sample diluent | MeCN/H2O -60/40% |
| LOD(Benzonitrile, Toluene, Benzylamine) | 27, 65, 103 ppb |
| Class of Compounds | Benzene, Aromatics |
| Analyzing Compounds | Benzonitrile,Toluene,Benzylamine |
HPLC Column Used
Primesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended