HPLC Separation of Acrylic Acid and 4- Methoxyphenol on Primesep B Column
https://sielc.com/hplc-separation-of-acrylic-acid-and-4-methoxyphenolChromatogram
Description
Acrylic acid in combination with other monomers produces copolymers which are used in manufacturing of various products such as paints, adhesives and plastics. Esters and salts of acrylic acid are known as acrylates. 4-methoxyphenol, also known as mequinol, is an active ingredient in topical drugs and is also used for inhibition of radical polymerization of monomers like acrylates. The two compounds can be separated on a mixed-mode Primesep B column with isocratic method in mobile phase of 20/80 Acetonitrile (ACN) and water with 0.02% phosphoric acid buffer. UV Detection 200nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H3PO4 – 0.02 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Antioxidant, Inhibitor, Phenol, Carboxylic acid |
| Analyzing Compounds | Acrylic Acid, 4- Methoxyphenol, Mequinol |
HPLC Column Used
Primesep B, 4.6×150 mm, 5 µm, 100A