HPLC Separation of Acrylamide and Melamine on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Acrylamide , Melamine .
Melamine is a nitrogen-based organic compound that was first synthesized by the German chemist Justus von Liebig in 1834. Melamine is a decorative coating that is resistant to water and mechanical damage.
Melamine is used for:
We were able to separate Melamine from Acrylamide in HPLC on Primesep 200 column using a mobile phase of water with Formic Acid buffer. This separation can be monitored with UV, ELSD, CAD detection techniques.
Method Parameters
| Mobile Phase | H2O – 99% |
|---|---|
| Buffer | Formic Acid – 1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 240 nm |
| Class of Compounds | Amide, Amine |
| Analyzing Compounds | Acrylamide,Melamine |
HPLC Column Used
Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended