HPLC Separation of 12 Pesticides on Newcrom B Column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine , Metalaxyl , Prochloraz , Paclobutrazol , Propanil , Chlorothalonil , Chlorpyrifos-methyl (Dursban) , Pentachlorophenol , Fenvalerate , Abamectin , Procymidone , Bifenazate .

6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C 12 H 11 N 5 . It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.

Metalaxyl , also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate,  is a systemic fungicide with the chemical formula C 15 H 21 NO 4 .  It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.

Prochloraz is an imidazole fungicide with the chemical formula C 15 H 16 Cl 3 N 3 O 2 . It is used widely across Europe, Asia, South America, and Australia, but is not registered for use in the United States. It works as an inhibitor of the enzyme lanosterol 14α-demethylase.

Paclobutrazol ( PBZ ) is a synthetic triazole compound with the chemical formula C 15 H 20 ClN 3 O . It is used to control plant growth as an antagonist of the plant hormone gibberellin. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants.

Procymidone is a pesticide and fungicide with the chemical formula C 13 H 11 Cl 2 NO 2 . It is typically used to kill unwanted ferns, nettles, and fungi in lupins, grapes, stone fruit, and strawberries.

Propanil is a contact herbicide with the chemical formula C 9 H 9 Cl 2 NO . It is one of the most widely used herbicides in America. It works as an inhibitor of photosynthesis and CO 2 fixation in weeds.

Method Parameters

HPLC Column Used