HPLC Method for Analysis of Benzoin on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Epibatidine
Benzoin is an organic compound with the chemical formula C 14 H 12 O 2 . It is primarily used as a precursor to benzil or in powder coating. You can find detailed UV spectra of Benzoin and information about its various lambda maxima by visiting the following link.
Benzoin can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/m |
| Detection | UV 200 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Benzoin |
HPLC Column Used
Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended