HPLC Method for Analysis of 4-Nitrobenzoyl Chloride on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Epibatidine
4-nitrobenzoyl chloride is an acyl halide with the chemical formula C 7 H 4 ClNO 3 . It is primarily used in preparation of polysubstituted furanonaphthoquinoines and as an intermediate in active pharmaceutical ingredients and dyes. You can find detailed UV spectra of 4-Nitrobenzoyl chloride and information about its various lambda maxima by visiting the following link.
4-Nitrobenzoyl chloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/m |
| Detection | UV 200 nm |
| Class of Compounds | Acyl Halide |
| Analyzing Compounds | 4-Nitrobenzoyl chloride |
HPLC Column Used
Primesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended