HPLC-MS Method for the Analysis of Tirzepatide in Pharmaceutical Formulation Mounjaro on Solid-Core Primesep SB Column
https://sielc.com/hplc-ms-method-for-analysis-tirzepatide-primesep-sbChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Tirzepatide
Tirzepatide is a synthetic peptide that functions as a dual agonist of the GLP-1 and GIP receptors. It is used in the treatment of type 2 diabetes and obesity. By mimicking the actions of both glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP), tirzepatide enhances insulin secretion, reduces blood sugar levels, promotes satiety, and aids in weight loss.
Tirzepatide can be retained and analyzed using the Solid-Core Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium formate. Detection was performed using UV absorption at 280 nm. Mass spectrometric detection was carried out in ESI-SIM mode, monitoring the ions [M+3H]³⁺ at m/z 1606
Method Parameters
| Mobile Phase | Gradient MeCN 40-80%, 10 min |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm, ESI-SIM: [M+3H]3⁺ -1606 |
| LOD | 8 ppb |
| Class of Compounds | GLP-1 Receptor Agonist |
| Analyzing Compounds | Tirzepatide |