HPLC-MS Method for Analysis of Semaglutide on Primesep SB Column
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Description
· HPLC Method for Analysis of Semaglutide on Primesep SB Column by SIELC Technologies
Semaglutide is a GLP-1 receptor agonist used for managing type 2 diabetes and obesity. It mimics the effects of the natural hormone GLP-1, promoting insulin secretion, reducing glucagon levels, slowing gastric emptying, and suppressing appetite. These mechanisms help control blood sugar levels and support weight loss.
Semaglutide can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and Ammonium formate. Detection was performed using UV absorption at 280 nm. Mass spectrometric detection was carried out in ESI-SIM mode, monitoring the ions [M+3H]³⁺ at m/z 1372 and [M+4H]⁴⁺ at m/z 1029
Method Parameters
| Mobile Phase | Gradient MeCN 40-80%, 10 min |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 280 nm, ESI-SIM: [M+3H]3⁺ -1372, [M+4H]4⁺ -1029 |
| LOD | 3 ppb |
| Class of Compounds | GLP-1 Receptor Agonist |
| Analyzing Compounds | Semaglutide |