HPLC Method for Analysis of Propranolol on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Propranolol · Propranolol is an organic compound with the molecular formula C 16 H 21 NO 2 . · Properties: Appearance: Typically a stable, odorless, white to off-white crystalline powder. · Molecular weight: ~259.34 g/mol · Solubility: Soluble in water and ethanol.
Uses: A non-selective beta-blocker. Used to treat a variety of conditions, both cardiovascular and non-cardiovascular.
Propranolol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 270 nm.
Method Parameters
| Mobile Phase | MeCN – 70% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270 nm |
| LOD | 14 ppb |
| Class of Compounds | Synthetic Organic Compound |
| Analyzing Compounds | Propranolol |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended