HPLC MS Method for Analysis of Streptomycin on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Streptomycin .
Streptomycin is the an antibiotic with the chemical formula C 21 H 39 N 7 O 12 . It is primarily used to treat bacterial infections but not limited to tuberculosis, endocarditis, brucellosis, and the plague. It is typically given intramuscularly. It works as a protein synthesis inhibitor by binding to the 16S rRNA of the 30S ribosomal subunit, which leads to it interfering with the binding of formyl-methionyl-tRNA. It is the official state microbe of New Jersey. You can find detailed UV spectra of Streptomycin and information about its various lambda maxima by visiting the following link.
Streptomycin can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using LC MS.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 0.5 ml/min |
| Detection | LC MS ESI SIM 582 (M+H)+ |
| LOD* | 0.2 ppm |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Streptomycin |