HPLC Method for Analysis of Diclofenac and Carbamazepine on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Diclofenac , Carbamazepine · Diclofenac is an organic compound with the molecular formula C 14 H 11 Cl 2 NO 2 .
Properties: Appearance: Typically an odorless, white to off-white crystalline, slightly hygroscopic powder.
Molecular weight: ~296.1 g/mol · Solubility: Soluble in organic solvents like ethanol.
Uses: A nonsteroidal anti-inflammatory drug (NSAID) used to relieve pain and reduce inflammation and swelling.
Carbamazepine is an organic compound with the molecular formula C 15 H 12 N 2 O .
Method Parameters
| Mobile Phase | MeCN – 45% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Diclofenac,Carbamazepine |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended