HPLC MS Method for Analysis of Benzalkonium Chloride on Primesep SB Column
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Description
· HPLC Method for Analysis of Benzalkonium chloride on Primesep B Column by SIELC Technologies
Benzalkonium chloride is a quaternary ammonium compound and belongs to the class of chemicals known as cationic surfactants. It is often used as a disinfectant, antiseptic, and preservative in various products, including pharmaceuticals, personal care products, and surface disinfectants.
Function: It has antimicrobial properties and is effective against bacteria, viruses, and fungi. It disrupts the cell membranes of microorganisms, leading to their inactivation.
Common Uses:
Variants: There are different variants of Benzalkonium chloride with varying alkyl chain lengths, and the specific composition may affect its properties and applications.
Benzalkonium can be retained, and analyzed using a Primesep SB mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using UV and LC MS positive mode
Method Parameters
| Mobile Phase | Gradient MeCN – 30 – 70%, 10 min |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 40 mM |
| Flow Rate | 0.2ml/min |
| Detection | UV 262 nm, SIM + 276, 304, 332, 360 |
| Samples | 0.0025 mg/mL in MeCN/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 1 ppb |
| Class of Compounds | Alkaloid, Quaternary ammonium |
| Analyzing Compounds | Benzalkonium chloride |