HPLC MS Method for Analysis of Amoxicillin on Primesep 100 Column
https://sielc.com/hplc-ms-method-for-analysis-amoxicillinChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of Amoxicillin on Primesep 100 Column by SIELC Technologies
Amoxicillin is a widely used antibiotic belonging to the penicillin class. It is used to treat a variety of bacterial infections, including respiratory tract infections, ear infections, skin infections, urinary tract infections, and certain types of bacterial endocarditis.
Amoxicillin can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 272 nm
Method Parameters
| Mobile Phase | MeCN 20% |
|---|---|
| Buffer | AmFm pH 3.0 – 15 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV 272nm, SIM + 349, 366 |
| Samples | 1 mg/ml |
| Injection volume | 5 µl |
| LOD* | 0.3 ppm |
| Class of Compounds | Drug |
| Analyzing Compounds | Amoxicillin |
HPLC Column Used
Primesep 100, 2.1 x 100 mm, 5 µm, 100 A, dual ended